In Vitro 3D

Despite of on-going research in tissue engineering approaches for the repair of the injured spinal cord, only recently have studies of cell-cell and cell-substrate interactions in 3D been performed (1, 2). It is known that electrospun fibres are capable of providing guidance for neurites in 2D and the guidance is improved by using adhesive/non-adhesive oriented substrates (3-5).

A) Dissociated and B) a pellet of U373 astrocytes seeded onto oriented solution electrospun fibers; C) Human mesenchymal stem cells encapsulated within a hyaluronic acid hydrogel without fibers and D) with melt electrospun fibers.
A) Dissociated and B) a pellet of U373 astrocytes seeded onto oriented solution electrospun fibers; C) Human mesenchymal stem cells encapsulated within a hyaluronic acid hydrogel without fibers and D) with melt electrospun fibers.

Advances in electrospinning have made it possible to deposit fibres with high levels of alignment (6,7). However, bringing aligned fibres into a 3D cell culture environment remains a challenge. The present investigation aims to develop a novel model to study the effect of aligned melt electrospun fibres on cell-cell and cell-substrate interactions in a 3D hydrogel environment.

References

  1. Wylie RG, Ahsan S, Aizawa Y, Maxwell KL, Morshead CM, Shoichet MS (2011) Spatially controlled simultaneous patterning of multiple growth factors in three-dimensional hydrogels. Nature Materials, 10, 799-806.
  2. Luo Y, Shoichet MS (2004) A photolabile hydrogel for guided 3-D cell growth and migration. Nature Materials, 3, 249-53.
  3. Schnell E, Klinkhammer K, Balzer S, Brook G, Klee D, Dalton PD, Mey J (2007) Guidance of glial cell migration and axonal growth on electrospun nanofibers of poly-e-caprolactone and a collagen/poly-e-caprolactone blend. Biomaterials, 28, 3012-3025.
  4. Gerardo Nava J, Klinkhammer K, Seiler N, Mey J, Klee D, Möller M, Dalton PD, Brook GA (2009) Neural and glial interactions with electrospun nanofibers in vitro. Nanomedicine4, 11-30.
  5. Klinkhammer K, Seiler N, Grafahrend D, Gerardo Nava J, Mey J, Brook GA, Möller M, Dalton PD, Klee D (2009) Deposition of electrospun fibers on reactive substrates for in vitro investigations. Tissue Eng Part C, 15, 77-85.
  6. Dalton PD, Klee D, Möller M (2005) Electrospinning with dual collection rings. Polymer, 46, 611-614.
  7. Brown TD, Dalton PD, Hutmacher DW. (2011) Direct Writing by Way of Melt Electrospinning. Advanced Materials, 23, 5651-57.