Despite of on-going research in tissue engineering approaches for the repair of the injured spinal cord, only recently have studies of cell-cell and cell-substrate interactions in 3D been performed (1, 2). It is known that electrospun fibres are capable of providing guidance for neurites in 2D and the guidance is improved by using adhesive/non-adhesive oriented substrates (3-5).
Advances in electrospinning have made it possible to deposit fibres with high levels of alignment (6,7). However, bringing aligned fibres into a 3D cell culture environment remains a challenge. The present investigation aims to develop a novel model to study the effect of aligned melt electrospun fibres on cell-cell and cell-substrate interactions in a 3D hydrogel environment.
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